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Rabbit anti-Collagen 2 alpha 1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Collagen 2 alpha 1

Synonyms

Collagen alpha-1 (II) chain; Alpha-1 type II collagen

Host

Rabbit

Reactivity

H, M, R, B, D, P, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 2 alpha 1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Collagen 2 alpha 1 expression in MCF7 (A) whole cell lysates.

IHC description

Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1280

Gene ID (Mouse)

12824

Gene ID (Rat)

25412

SwissProt (Human)

P02458

SwissProt (Mouse)

P28481

SwissProt (Rat)

P05539

CAS Number

9007-83-4

Available Sizes

Curated Selection

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