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Rabbit anti-c-Jun Antibody

Product Specifications

Background

Rabbit polyclonal antibody to c-Jun

Synonyms

Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

Host

Rabbit

Reactivity

H, M, R, B, P, Rb, S

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of c-Jun expression in HEK293T (A), SP2/0 (B), H9C2 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

3725

Gene ID (Mouse)

16476

Gene ID (Rat)

24516

SwissProt (Human)

P05412

SwissProt (Mouse)

P05627

SwissProt (Rat)

P17325

CAS Number

9007-83-4

Available Sizes

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