Rabbit anti-c-Jun Antibody
Product Specifications
Background
Rabbit polyclonal antibody to c-Jun
CAS Number
9007-83-4
Synonyms
JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUND; Transcription factor jun-D
Host
Rabbit
Reactivity
H, M, R, B, C, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of c-Jun expression in Jurkat (A), HepG2 (B), MCF7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3725; 3727
Gene ID (Mouse)
16476; 16478
Gene ID (Rat)
24516; 24518
SwissProt (Human)
P05412; P17535
SwissProt (Mouse)
P05627; P15066
SwissProt (Rat)
P17325; P52909
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