Rabbit anti-Citrate Synthase Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Citrate Synthase
Synonyms
Citrate synthase, mitochondrial; Citrate (Si) -synthase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Citrate Synthase
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Citrate Synthase expression in HepG2 (A), MCF7 (B), mouse liver (C), rat testis (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Citrate Synthase staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1431
Gene ID (Mouse)
12974
Gene ID (Rat)
170587
SwissProt (Human)
O75390
SwissProt (Mouse)
Q9CZU6
SwissProt (Rat)
Q8VHF5
CAS Number
9007-83-4
Available Sizes
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