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Rabbit anti-CHK2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CHK2

Synonyms

CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Host

Rabbit

Reactivity

H, M, R, B, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHK2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CHK2 expression in MCF7 (A), Jurkat (B) whole cell lysates.

IHC description

Immunohistochemical analysis of CHK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

11200

Gene ID (Mouse)

50883

SwissProt (Human)

O96017

SwissProt (Mouse)

Q9Z265

CAS Number

9007-83-4

Available Sizes

Curated Selection

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