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Rabbit anti-CHK1 (pS301) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CHK1 (pS301)

CAS Number

9007-83-4

Synonyms

CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHK1 (pS301) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CHK1 (pS301) expression in HEK293T (A) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CHK1 (pS301) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1111

Gene ID (Mouse)

12649

Gene ID (Rat)

140583

SwissProt (Human)

O14757

SwissProt (Mouse)

O35280

SwissProt (Rat)

Q91ZN7

Available Sizes

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