Rabbit anti-CDC5L Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CDC5L
Synonyms
KIAA0432; PCDC5RP; Cell division cycle 5-like protein; Cdc5-like protein; Pombe cdc5-related protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CDC5L
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CDC5L expression in MCF7 (A), K562 (B), mouse testis (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CDC5L staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
988
Gene ID (Mouse)
71702
Gene ID (Rat)
85434
SwissProt (Human)
Q99459
SwissProt (Mouse)
Q6A068
SwissProt (Rat)
O08837
CAS Number
9007-83-4
Available Sizes
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