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Rabbit anti-CDC5L Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CDC5L

CAS Number

9007-83-4

Synonyms

KIAA0432; PCDC5RP; Cell division cycle 5-like protein; Cdc5-like protein; Pombe cdc5-related protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CDC5L

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CDC5L expression in MCF7 (A), K562 (B), mouse testis (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CDC5L staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

988

Gene ID (Mouse)

71702

Gene ID (Rat)

85434

SwissProt (Human)

Q99459

SwissProt (Mouse)

Q6A068

SwissProt (Rat)

O08837

Available Sizes

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