Rabbit anti-CDC34B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CDC34B

CAS Number

9007-83-4

Synonyms

CDC34B; UBC3B; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CDC34B

Clonality

Polyclonal

Applications

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CDC34B expression in Jurkat (A), MCF7 (B), mouse liver (C), mouse kidney (D), rat brain (E), rat lung (F) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CDC34B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.