Rabbit anti-CDC25A (pS178) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CDC25A (pS178)

CAS Number

9007-83-4

Synonyms

M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25A. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CDC25A (pS178) expression in HEK293T UV-treated (A), HeLa UV-treated (B) whole cell lysates.

IHC description

Immunohistochemical analysis of CDC25A (pS178) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.