Rabbit anti-CD27 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CD27
Synonyms
TNFRSF7; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD27
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CD27
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CD27 expression in Raji (A), HL60 (B), mouse skeletal muscle (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CD27 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
939
Gene ID (Mouse)
21940
SwissProt (Human)
P26842
SwissProt (Mouse)
P41272
CAS Number
9007-83-4
Available Sizes
Curated Selection
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