Rabbit anti-CASK Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CASK

CAS Number

9007-83-4

Synonyms

LIN2; Peripheral plasma membrane protein CASK; hCASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CASK

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CASK expression in HEK293T (A), H460 (B), A549 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CASK staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

8573

Gene ID (Mouse)

12361

Gene ID (Rat)

29647

SwissProt (Human)

O14936

SwissProt (Mouse)

O70589

SwissProt (Rat)

Q62915

Available Sizes

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