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Rabbit anti-CARK Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CARK

CAS Number

9007-83-4

Synonyms

CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARK. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CARK expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

100526835; 51086

Gene ID (Mouse)

435766

Gene ID (Rat)

295531

SwissProt (Human)

Q59H18

SwissProt (Mouse)

Q5GIG6

SwissProt (Rat)

Q7TQP6

Available Sizes

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