What is the catalog number for Rabbit anti-CARD14 Antibody?

The catalog number for Rabbit anti-CARD14 Antibody is YLD1146.

What sizes are available for Rabbit anti-CARD14 Antibody?

Rabbit anti-CARD14 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-CARD14 Antibody come in?

Rabbit anti-CARD14 Antibody is available in 2 different pack sizes.

Is Rabbit anti-CARD14 Antibody in stock?

Rabbit anti-CARD14 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-CARD14 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CARD14

CAS Number

9007-83-4

Synonyms

CARMA2; Caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; Carma 2

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARD14. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CARD14 expression in HeLa (A) whole cell lysates.

IHC description

Immunohistochemical analysis of CARD14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of CARD14 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .