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Rabbit anti-CapZ alpha-2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CapZ alpha-2

CAS Number

9007-83-4

Synonyms

F-actin-capping protein subunit alpha-2; CapZ alpha-2

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CapZ alpha-2

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of CapZ alpha-2 expression in Hela (A), THP1 (B), mouse brain (C), mouse heart (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CapZ alpha-2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

830

Gene ID (Mouse)

12343

Gene ID (Rat)

493810

SwissProt (Human)

P47755

SwissProt (Mouse)

P47754

SwissProt (Rat)

Q3T1K5

Available Sizes

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