Rabbit anti-Calpain 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Calpain 1
Synonyms
CANPL1; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Calpain 1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Calpain 1 expression in A549 (A), MCF7 (B), Jurkat (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Calpain 1 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
823
Gene ID (Mouse)
12333
Gene ID (Rat)
29153
SwissProt (Human)
P07384
SwissProt (Mouse)
O35350
SwissProt (Rat)
P97571
CAS Number
9007-83-4
Available Sizes
Curated Selection
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