Rabbit anti-C/EBP alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to C/EBP alpha
Synonyms
CCAAT/enhancer-binding protein alpha; C/EBP alpha
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human C/EBP alpha. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of C/EBP alpha expression in mouse embryo (A), rat ovary (B), NIH3T3L1 (C), PC12 (D), Hela (E), Panc1 (F), SGC7901 (G) whole cell lysates.
IHC description
Immunohistochemical analysis of C/EBP alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1050
Gene ID (Mouse)
12606
Gene ID (Rat)
24252
SwissProt (Human)
P49715
SwissProt (Mouse)
P53566
SwissProt (Rat)
P05554
CAS Number
9007-83-4
Available Sizes
Curated Selection
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