Rabbit anti-BTEB1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BTEB1
Synonyms
BTEB; BTEB1; Krueppel-like factor 9; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human BTEB1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BTEB1 expression in mouse lung (A), mouse liver (B), rat brain (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of BTEB1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
687
Gene ID (Rat)
117560
SwissProt (Human)
Q13886
SwissProt (Mouse)
O35739
SwissProt (Rat)
Q01713
CAS Number
9007-83-4
Available Sizes
Curated Selection
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