What is the catalog number for Rabbit anti-BRCA1 Antibody?

The catalog number for Rabbit anti-BRCA1 Antibody is YLD0960.

What sizes are available for Rabbit anti-BRCA1 Antibody?

Rabbit anti-BRCA1 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-BRCA1 Antibody come in?

Rabbit anti-BRCA1 Antibody is available in 2 different pack sizes.

Is Rabbit anti-BRCA1 Antibody in stock?

Rabbit anti-BRCA1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-BRCA1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to BRCA1

CAS Number

9007-83-4

Synonyms

RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRCA1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of BRCA1 expression in K562 (A), HEK293T (B), HT29 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of BRCA1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.