Rabbit anti-BORIS Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BORIS
Synonyms
BORIS; Transcriptional repressor CTCFL; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein; Cancer/testis antigen 27; CT27; Zinc finger protein CTCF-T
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human BORIS
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BORIS expression in Jurkat (A), Hela (B), mouse heart (C), rat testis (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of BORIS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
140690
Gene ID (Mouse)
664799
SwissProt (Human)
Q8NI51
SwissProt (Mouse)
A2APF3
CAS Number
9007-83-4
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
