Rabbit anti-Beta-actin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Beta-actin
CAS Number
9007-83-4
Synonyms
Actin cytoplasmic 1; Beta-actin
Host
Rabbit
Reactivity
H, M, R, B, C, D, Mk, P, Rb, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Beta-actin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of Beta-actin expression in Jurkat (A), HeLa (B), NIH3T3 (C), rat liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of Beta-actin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
60
Gene ID (Mouse)
11461
Gene ID (Rat)
81822
SwissProt (Human)
P60709
SwissProt (Mouse)
P60710
SwissProt (Rat)
P60711
Available Sizes
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