Rabbit anti-BARD1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BARD1
Synonyms
BRCA1-associated RING domain protein 1; BARD-1
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BARD1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BARD1 expression in MCF7 (A), HeLa (B) whole cell lysates.
IHC description
Immunohistochemical analysis of BARD1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
580
SwissProt (Human)
Q99728
CAS Number
9007-83-4
Available Sizes
Curated Selection
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