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Rabbit anti-B4GALT3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to B4GALT3

Synonyms

Beta-1,4-galactosyltransferase 3; Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human B4GALT3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of B4GALT3 expression in Jurkat (A), HL60 (B), HeLa (C), COLO205 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of B4GALT3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

8703

Gene ID (Mouse)

57370

Gene ID (Rat)

494342

SwissProt (Human)

O60512

SwissProt (Mouse)

Q91YY2

SwissProt (Rat)

Q6P768

CAS Number

9007-83-4

Available Sizes

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