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Rabbit anti-ATPIF1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATPIF1

Synonyms

ATPI; ATPase inhibitor, mitochondrial; Inhibitor of F (1) F (o) -ATPase; IF (1) ; IF1

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human ATPIF1

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ATPIF1 expression in MCF7 (A), Hela (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of ATPIF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

93974

Gene ID (Mouse)

11983

SwissProt (Human)

Q9UII2

SwissProt (Mouse)

O35143

CAS Number

9007-83-4

Available Sizes

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