Rabbit anti-ATP5I Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ATP5I
Synonyms
ATP5K; ATP synthase subunit e, mitochondrial; ATPase subunit e
Host
Rabbit
Reactivity
H, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP5I. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ATP5I expression in RT4 (A), COLO205 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of ATP5I staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
521
SwissProt (Human)
P56385
CAS Number
9007-83-4
Available Sizes
Curated Selection
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