Rabbit anti-Atlastin-1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Atlastin-1

Synonyms

GBP3; SPG3A; Atlastin-1; Brain-specific GTP-binding protein; GTP-binding protein 3; GBP-3; hGBP3; Guanine nucleotide-binding protein 3; Spastic paraplegia 3 protein A

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Atlastin-1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Atlastin-1 expression in LOVO (A), HEK293T (B), mouse brain (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Atlastin-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

51062

Gene ID (Mouse)

73991

Gene ID (Rat)

362750

SwissProt (Human)

Q8WXF7

SwissProt (Mouse)

Q8BH66

SwissProt (Rat)

Q6PST4

CAS Number

9007-83-4

Available Sizes

Curated Selection

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