Rabbit anti-Atlastin-1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Atlastin-1
Synonyms
GBP3; SPG3A; Atlastin-1; Brain-specific GTP-binding protein; GTP-binding protein 3; GBP-3; hGBP3; Guanine nucleotide-binding protein 3; Spastic paraplegia 3 protein A
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Atlastin-1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Atlastin-1 expression in LOVO (A), HEK293T (B), mouse brain (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Atlastin-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
51062
Gene ID (Mouse)
73991
Gene ID (Rat)
362750
SwissProt (Human)
Q8WXF7
SwissProt (Mouse)
Q8BH66
SwissProt (Rat)
Q6PST4
CAS Number
9007-83-4
Available Sizes
Curated Selection
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