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Rabbit anti-ATG5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATG5

CAS Number

9007-83-4

Synonyms

APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human ATG5

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of ATG5 expression in Hela (A), NIH3T3 (B), PC12 (C), rat bain (D) whole cell lysates.

IHC description

Immunohistochemical analysis of ATG5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

9474

Gene ID (Mouse)

11793

SwissProt (Human)

Q9H1Y0

SwissProt (Mouse)

Q99J83

SwissProt (Rat)

Q3MQ06

Available Sizes

Frequently Asked Questions

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