Rabbit anti-ATG4D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ATG4D
CAS Number
9007-83-4
Synonyms
APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of ATG4D expression in Hela (A), A549 (B), PC12 (C), CT26 (D) whole cell lysates.
IHC description
Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
84971
Gene ID (Mouse)
235040
SwissProt (Human)
Q86TL0
SwissProt (Mouse)
Q8BGV9
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