Rabbit anti-ATF6 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ATF6
Synonyms
Cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide of human ATF6
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ATF6 expression in K562 (A), PC3 (B), mouse heart (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of ATF6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
22926
Gene ID (Mouse)
226641
Gene ID (Rat)
304962
SwissProt (Human)
P18850
SwissProt (Mouse)
F6VAN0
SwissProt (Rat)
G3V909
CAS Number
9007-83-4
Available Sizes
Curated Selection
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