Rabbit anti-ATF4 (pS245) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ATF4 (pS245)
Synonyms
CREB2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATF4 (pS245) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ATF4 (pS245) expression in MCF7 (A), mouse heart (B), rat heart (C) whole cell lysates.
IHC description
Immunohistochemical analysis of ATF4 (pS245) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
468
Gene ID (Mouse)
11911
Gene ID (Rat)
79255
SwissProt (Human)
P18848
SwissProt (Mouse)
Q06507
SwissProt (Rat)
Q9ES19
CAS Number
9007-83-4
Available Sizes
Curated Selection
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