Rabbit anti-AREB6 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to AREB6
Synonyms
AREB6; TCF8; Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human AREB6
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of AREB6 expression in SKOV3 (A), Hela (B), NIH3T3 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of AREB6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6935
Gene ID (Mouse)
21417
SwissProt (Human)
P37275
SwissProt (Mouse)
Q64318
SwissProt (Rat)
Q62947
CAS Number
9007-83-4
Available Sizes
Curated Selection
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