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Rabbit anti-ANT4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ANT4

CAS Number

9007-83-4

Synonyms

AAC4; ANT4; SFEC; ADP/ATP translocase 4; ADP, ATP carrier protein 4; Adenine nucleotide translocator 4; ANT 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein

Host

Rabbit

Reactivity

H, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANT4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of ANT4 expression in K562 (A), U251MG (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ANT4 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

83447

SwissProt (Human)

Q9H0C2

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