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Rabbit anti-Annexin A11 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Annexin A11

CAS Number

9007-83-4

Synonyms

ANX11; Annexin A11; 56 kDa autoantigen; Annexin XI; Annexin-11; Calcyclin-associated annexin 50; CAP-50

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Annexin A11

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of Annexin A11 expression in Jurkat (A), SKOV3 (B), A549 (C), mouse kidney (D), rat brain (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Annexin A11 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

311

Gene ID (Mouse)

11744

SwissProt (Human)

P50995

SwissProt (Mouse)

P97384

Available Sizes

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