Rabbit anti-AMBRA1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to AMBRA1
Synonyms
KIAA1736; Activating molecule in BECN1-regulated autophagy protein 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human AMBRA1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
55626
Gene ID (Mouse)
228361
SwissProt (Human)
Q9C0C7
SwissProt (Mouse)
A2AH22
CAS Number
9007-83-4
Available Sizes
Curated Selection
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