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Rabbit anti-Alpha-tubulin 4a Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Alpha-tubulin 4a

Synonyms

TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Alpha-tubulin 4a

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/25 - 1/50), IF/IC (1/25 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Alpha-tubulin 4a expression in HepG2 (A), MCF7 (B), mouse heart (C), mouse brain (D), PC12 (E) whole cell lysates.

IHC description

Immunohistochemical analysis of Alpha-tubulin 4a staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Alpha-tubulin 4a staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

7277

Gene ID (Mouse)

22145

Gene ID (Rat)

316531

SwissProt (Human)

P68366

SwissProt (Mouse)

P68368

SwissProt (Rat)

Q5XIF6

CAS Number

9007-83-4

Available Sizes

Curated Selection

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