Rabbit anti-Alpha-tubulin 4a Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Alpha-tubulin 4a
Synonyms
TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Alpha-tubulin 4a
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-tubulin 4a expression in LOVO (A), HEK293T (B), HUVEC (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Alpha-tubulin 4a staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
7277
Gene ID (Mouse)
22145
Gene ID (Rat)
316531
SwissProt (Human)
P68366
SwissProt (Mouse)
P68368
SwissProt (Rat)
Q5XIF6
CAS Number
9007-83-4
Available Sizes
Curated Selection
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