Rabbit anti-Alpha-enolase Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Alpha-enolase
Synonyms
ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Alpha-enolase
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-enolase expression in PC3 (A), Ramos (B), Hela (C), THP1 (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Alpha-enolase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2023
Gene ID (Mouse)
13806; 433182
Gene ID (Rat)
24333
SwissProt (Human)
P06733
SwissProt (Mouse)
P17182
SwissProt (Rat)
P04764
CAS Number
9007-83-4
Available Sizes
Curated Selection
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