Rabbit anti-Alpha-crystallin C Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Alpha-crystallin C
Synonyms
CRYAC; E2IG1; HSP22; Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Alpha-crystallin C
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-crystallin C expression in BT474 (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Alpha-crystallin C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Alpha-crystallin C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
26353
Gene ID (Mouse)
80888
Gene ID (Rat)
113906
SwissProt (Human)
Q9UJY1
SwissProt (Mouse)
Q9JK92
SwissProt (Rat)
Q9EPX0
CAS Number
9007-83-4
Available Sizes
Curated Selection
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