Rabbit anti-Alpha-actin-1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Alpha-actin-1

CAS Number

9007-83-4

Synonyms

ACTA; Actin, alpha skeletal muscle; Alpha-actin-1

Host

Rabbit

Reactivity

H, M, R, B, C, P, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin-1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of Alpha-actin-1 expression in A549 (A), SP2/0 (B), rat kidney (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Alpha-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunoprecipitation of Alpha-actin-1 from 0.5mg Mouse Skeletal Muscle tissue lysate, using 5ug of anti-Alpha-actin-1 Antibody and 50ul of protein G magnetic beads (+) . No antibody was added to the control (-) . The antibody was incubated under agitation with Protein G beads for 10min, Mouse Skeletal Muscle tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-Alpha-actin-1 Antibody.