Rabbit anti-Alpha-2A Adrenergic Receptor Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Alpha-2A Adrenergic Receptor
Synonyms
ADRA2R; ADRAR; Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-2A Adrenergic Receptor. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-2A Adrenergic Receptor expression in HepG2 (A), mouse heart (B), rat heart (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Alpha-2A Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
150
SwissProt (Human)
P08913
CAS Number
9007-83-4
Available Sizes
Curated Selection
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