Rabbit anti-ALAS-E Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ALAS-E
Synonyms
ALASE; ASB; 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human ALAS-E
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ALAS-E expression in HEK293T (A), mouse lung (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of ALAS-E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
212
Gene ID (Mouse)
11656
Gene ID (Rat)
25748
SwissProt (Human)
P22557
SwissProt (Mouse)
P08680
SwissProt (Rat)
Q63147
CAS Number
9007-83-4
Available Sizes
Curated Selection
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