Rabbit anti-AKT (pT450) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to AKT (pT450)
Synonyms
PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Host
Rabbit
Reactivity
H, M, R, B, P, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of AKT (pT450) expression in HeLa (A), HepG2 colchicine-treated (B), NIH3T3 UV-treated (C), PC12 H2O2-treated (D) whole cell lysates.
IHC description
Immunohistochemical analysis of AKT (pT450) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
207
Gene ID (Mouse)
11651
Gene ID (Rat)
24185
SwissProt (Human)
P31749
SwissProt (Mouse)
P31750
SwissProt (Rat)
P47196
CAS Number
9007-83-4
Available Sizes
Curated Selection
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