Rabbit anti-ADH5 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ADH5
CAS Number
9007-83-4
Synonyms
ADHX; FDH; Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S- (hydroxymethyl) glutathione dehydrogenase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human ADH5
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of ADH5 expression in U251 (A), U937 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of ADH5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
128
Gene ID (Mouse)
11532
Gene ID (Rat)
100145871
SwissProt (Human)
P11766
SwissProt (Mouse)
P28474
SwissProt (Rat)
P12711
Available Sizes
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