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Rabbit anti-Adenylate Cyclase 5/6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Adenylate Cyclase 5/6

Synonyms

ADCY5; Adenylate cyclase type 5; ATP pyrophosphate-lyase 5; Adenylate cyclase type V; Adenylyl cyclase 5; ADCY6; KIAA0422; Adenylate cyclase type 6; ATP pyrophosphate-lyase 6; Adenylate cyclase type VI; Adenylyl cyclase 6; Ca (2+) -inhibitable adenylyl cyclase

Host

Rabbit

Reactivity

H, M, R, D, Mk, Rb, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenylate Cyclase 5/6. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat brain (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Adenylate Cyclase 5/6 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

111; 112

Gene ID (Mouse)

224129; 11512

Gene ID (Rat)

64532

SwissProt (Human)

O95622; O43306

SwissProt (Mouse)

P84309; Q01341

SwissProt (Rat)

Q04400; Q03343

CAS Number

9007-83-4

Available Sizes

Curated Selection

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