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Rabbit anti-ACAT1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ACAT1

Synonyms

ACAT; MAT; Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; T2

Host

Rabbit

Reactivity

H, M, R, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACAT1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ACAT1 expression in HeLa (A), HEK293T (B), SP2/0 (C), H9C2 (D), THP1 (E) whole cell lysates.

IHC description

Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

38

Gene ID (Mouse)

110446

Gene ID (Rat)

25014

SwissProt (Human)

P24752

SwissProt (Mouse)

Q8QZT1

SwissProt (Rat)

P17764

CAS Number

9007-83-4

Available Sizes

Curated Selection

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