Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-AAK1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to AAK1

CAS Number

9007-83-4

Synonyms

KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AAK1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of AAK1 expression in HepG2 (A), COS7 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of AAK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of AAK1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

22848

Gene ID (Mouse)

269774

SwissProt (Human)

Q2M2I8

SwissProt (Mouse)

Q3UHJ0

SwissProt (Rat)

P0C1X8

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

PTP epsilon (PTPRE) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3E1]
TA501233AM 100 µL

PTP epsilon (PTPRE) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3E1]

Ask
View Details
SOCS3 Antibody
A35364-100UG 100 µg

SOCS3 Antibody

Ask
View Details
Rabbit Polyclonal PSF2 Antibody
NBP1-58208 100 µL

Rabbit Polyclonal PSF2 Antibody

Ask
View Details
Rabbit anti-Escherichia coli (strain K12) mdoC Polyclonal Antibody
MBS7148313 Inquire

Rabbit anti-Escherichia coli (strain K12) mdoC Polyclonal Antibody

Ask
View Details