Rabbit anti-4EBP1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to 4EBP1

CAS Number

9007-83-4

Synonyms

EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Host

Rabbit

Reactivity

H, M, R, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 4EBP1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of 4EBP1 expression in HeLa (A), MCF7 (B), mouse kidney (C), rat liver (D) whole cell lysates.

IHC description

Immunohistochemical analysis of 4EBP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.