Rabbit anti-4EBP1 (pT46) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to 4EBP1 (pT46)
Synonyms
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Host
Rabbit
Reactivity
H, M, R, B, P, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 4EBP1 (pT46) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of 4EBP1 (pT46) expression in K562 PMA-treated (A), NIH3T3 PMA-treated (B), PC12 PMA-treated (C) whole cell lysates.
IHC description
Immunohistochemical analysis of 4EBP1 (pT46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1978
Gene ID (Mouse)
13685
Gene ID (Rat)
116636
SwissProt (Human)
Q13541
SwissProt (Mouse)
Q60876
SwissProt (Rat)
Q62622
CAS Number
9007-83-4
Available Sizes
Curated Selection
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