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Rabbit anti-14-3-3 theta/tau Antibody

Product Specifications

Background

Rabbit polyclonal antibody to 14-3-3 theta/tau

Synonyms

14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

Host

Rabbit

Reactivity

H, M, R, B, C, P, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 theta/tau. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of 14-3-3 theta/tau expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of 14-3-3 theta/tau staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of 14-3-3 theta/tau staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

10971

Gene ID (Mouse)

102634437; 22630

Gene ID (Rat)

25577

SwissProt (Human)

P27348

SwissProt (Mouse)

P68254

SwissProt (Rat)

P68255

CAS Number

9007-83-4

Available Sizes

Curated Selection

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