Rabbit anti-14-3-3 epsilon Antibody
Product Specifications
Background
Rabbit polyclonal antibody to 14-3-3 epsilon
Synonyms
14-3-3 protein epsilon; 14-3-3E
Host
Rabbit
Reactivity
M, R
Immunogen
Recombinant protein corresponding to mouse 14-3-3 epsilon.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of 14-3-3 epsilon expression in mouse brain (A), rat brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Mouse)
22627
Gene ID (Rat)
29753
SwissProt (Mouse)
P62259
SwissProt (Rat)
P62260
CAS Number
9007-83-4
Available Sizes
Curated Selection
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