Rabbit anti-14-3-3 epsilon Antibody

Product Specifications

Background

Rabbit polyclonal antibody to 14-3-3 epsilon

CAS Number

9007-83-4

Synonyms

14-3-3 protein epsilon; 14-3-3E

Host

Rabbit

Reactivity

M, R

Immunogen

Recombinant protein corresponding to mouse 14-3-3 epsilon.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of 14-3-3 epsilon expression in mouse brain (A), rat brain (B) whole cell lysates.

IHC description

Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.